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Methylene Blue IV (M-Blue): A Powerful Antioxidant and Versatile Medication


Methylene Blue (M-Blue) is an antioxidant substance with bioactive properties that aid in regenerating and repairing mitochondria, enabling them to function correctly. This potent nutraceutical can cross the Blood Brain Barrier to delay cellular aging and enhance mitochondrial biochemical pathways.

Initially, Methylene Blue was approved by the FDA for treating methemoglobinemia. For decades, it has been used for various conventional medical purposes in hospitals and for other medical applications. At Sponaugle Wellness, we use Methylene Blue both orally and intravenously to treat several conditions, including Lyme disease, babesia, and anaplasma.

Methylene Blue IV has demonstrated promising results in killing parasites, enhancing mitochondrial function, and protecting the brain against disease while simultaneously enhancing nerve cell signaling.

History of Methylene Blue IV

Methylene Blue is a medication that has been around for a long time and predates all other pharmaceuticals on the market. Psychiatrists used Methylene Blue in the early 20th century to experimentally treat schizophrenia.

Originally developed as an antimalarial drug by Paul Guttmann and Paul Ehrlich in 1891, Methylene Blue was used to prevent and treat malaria during World War II. It still outperforms other synthetic antimalarial pharmaceuticals for treatment.

In 1891, a German physician and Nobel Prize recipient discovered that when Methylene Blue was injected into animals in the lab, it would quickly concentrate in the brain and selectively target diseased tissues in the body. Ehrlich coined the term “Magic Bullet” for this unique action displayed by Methylene Blue, a term still used today.

Methylene Blue has been used to treat various conditions, including dementia, cancer chemotherapy, urinary tract infections, cyanide, and carbon monoxide poisoning.

Mechanisms of Action for Methylene Blue: Boosting Brain Function and ATP Production

Methylene Blue is a crucial agent in anti-aging and neurological disease prevention, especially for the brain. The brain’s neurons rely almost entirely on mitochondrial energy, and failure of mitochondrial function can cause severe damage to the brain and the rest of the body.

Methylene Blue improves memory by increasing how the brain cell utilizes oxygen, known as brain cell respiration. It also increases ATP production, providing more cellular energy for better overall brain function, including cognition, mood, and memory.

This compound quickly crosses the blood-brain barrier, improving mitochondrial efficiency and respiration, acting as an antioxidant, and increasing brain cell lifespan. By acting as an electron donor to the mitochondria, it increases ATP production, which is essential to power human life. Even healthy individuals can benefit from a boost in ATP production, as declining ATP levels can lead to a decrease in both physical and mental performance.

Methylene Blue’s ability to increase the amount of NAD+ produced by the mitochondria also offers further benefits to brain function and overall health.


Impact of Methylene Blue on Neurotransmitters Methylene Blue has been observed to restrict the activity of monoamine oxidase and acetylcholinesterase, increasing catecholamine and acetylcholine levels. Additionally, it also enhances serotonin and norepinephrine, which can affect memory, anxiety, and depression.


Methylene Blue: A Powerful Antioxidant
Mitochondria produce reactive oxygen species, with superoxide being the first free radical created within a cell. Fortunately, Methylene Blue (MB) has the ability to bind to superoxide and convert it into water, thus halting the oxidative cascade before it can cause any harm. This makes MB an effective antioxidant.

Methylene Blue: Benefits for Memory and Cognitive Function
Methylene Blue has been shown to increase brain cell respiration, which aids in memory improvement. By increasing ATP production, cellular energy is increased resulting in an overall improvement in brain function, including cognition, mood and memory.

Methylene Blue: Antioxidant

Moreover, Methylene Blue acts as an antioxidant by binding to superoxide and reducing it to water, thus halting the oxidative cascade before it can cause harm.

Methylene Blue resists Alzheimer’s Disease.

Methylene Blue: A Promising Treatment for Alzheimer’s and Dementia
Alzheimer’s disease and other forms of dementia are associated with a protein called Tau, which leads to the formation of amyloid plaques and neurofibrillary tangles. However, clinical trials have demonstrated that Methylene Blue can successfully inhibit Tau formation, making it a potential treatment for Alzheimer’s.

Methylene Blue has an inhibitory effect on the cGMP pathway and other molecular events closely related to Alzheimer’s progression. Additionally, it improves neuron resistance to the formation of amyloid plaques and neurofibrillary tangles. Furthermore, it helps repair impairments in mitochondrial function and cellular metabolism.

Research shows that the cholinergic, serotonergic, and glutamatergic systems all play a significant role in the development of Alzheimer’s and other cognitive disorders. Methylene Blue provides beneficial effects in mediating these pathways.

What’s even more promising is that Methylene Blue has the potential to delay the effects of Alzheimer’s and dementia after it has been diagnosed. This is significant because most existing treatments can only prevent the disease before it is diagnosed.

Methylene Blue as an Antidepressant: Understanding its Mechanism
Methylene Blue functions as a monoamine oxidase inhibitor (MAOI), with a greater impact on MAO-A than MAO-B. However, at higher doses, it inhibits both. In 1987, a study demonstrated that 15 mg of Methylene Blue daily had a powerful antidepressant effect on individuals with severe depression. In another study, 31 Bipolar Disorder patients who were also taking lithium were given either 300 mg or 15 mg of Methylene Blue per day. The findings indicated that the 300 mg dose of Methylene Blue was a valuable adjunct to lithium for the long-term treatment of manic-depressive psychosis, with patients experiencing significantly less depression.

Methylene Blue boosts acetylcholine.

According to research, Methylene Blue acts as an acetylcholinesterase inhibitor, with a particular affinity for muscarinic acetylcholine receptors. This means that MB can impede the breakdown of acetylcholine, allowing more to be available in the brain. [vii]

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